
Looks like I am not the only one who has a virus at the moment.
The trusty old Osborne ended up with what appeared to be an MBR or boot-sector infection.
The Sound Blaster 16 installation disks I had been using were also detected as infected.
At the time I assumed those disks had infected the PC.
I cannot actually prove the direction of transmission though. Without a known-clean copy of the disks from before they entered the Osborne, the PC could just as easily have infected them.

I only discovered the problem after using the disks in the Osborne and then bringing them back to another machine to reformat and re-image.
So a big thanks to Avast under Windows XP for sounding the alarm, and to Caluser2000 over at the Vintage Computer forums for pointing me towards a DOS-era cleanup tool.

F-PROT 3.16f saved the day.
The archive I used was fprot_dos_316f.zip, with virus definitions dating into 2009.
F-PROT 3.16f was the final DOS version and is perfectly at home on a 486.
For this sort of boot-sector cleanup, the important part is starting the infected computer from known-clean media rather than booting from the compromised hard disk.
Ideally that rescue floppy should also be write-protected so the infected system cannot modify it.
Windows 95 is now installed as well, and the next challenge is getting this thing onto the internet to some degree.
Related posts
Sources
- DOS Days - Antivirus Utilities - documents F-PROT 3.16f as the final DOS release and preserves historical usage details.